The influence of the initial fatigue loading on tensile characteristics of the TiAl6V4 titanium alloy is presented in this work. For cumulative fatigue damage equal to 0.75, a decrease in the pre-fatigue amplitude leads to a lowering in the elongation. Moreover, independent of the amplitude, the loading induces an increase in the yield stress by approximately 100 MPa. An increase in the number of pre-fatigue cycles at a constant amplitude results in a decrease in the elongation. Such mechanical behaviour is related to the cyclic hardening effect and the development of fatigue damage, which clearly affects the tensile characteristics of the alloy.