Since 1963
ARTICLE
Effects of X-ray on fibroblast mechanical properties
1
Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran
2
Department of Basic Sciences of Rehabilitation, Iran University of Medical Sciences (IUMS)
Submission date: 2018-09-03
Acceptance date: 2019-05-31
Online publication date: 2019-10-15
Publication date: 2019-10-15
Journal of Theoretical and Applied Mechanics 2019;57(4):999-1008
KEYWORDS
ABSTRACT
Concerning the widespread use of X-rays to detect various diseases, such as oral and dental
ones, it is essential to study the effects of this radiation on living cells. From the past, ge-
netic effects and cell death because of X-rays have been studied. In addition, the effect of
this ionizing radiation on the mechanical properties of the cell and cytoskeleton has been
studied, but different results have been obtained based on different models. In this study,
post-culture gingival fibroblast cells were classified into two groups of control and radiation
with Nano Magnetic Particles functionalized by folic acid. The cells of the radiation group
were exposed to X-rays of 3mGy·cm2. The specimens were undergone static creep test by
a magnetic tweezer. Spring and damper coefficients were obtained based on the viscoelastic
solid modeling. The static and dynamic stiffness of the groups was also calculated. The ma-
ximum deformation was decreased after radiation from 0.049 ± 0.01 μm to 0.029 ± 0.01 μm
and the static stiffness of the model was 1.6 times decreased. Also, the gel point frequency
for the control group was 27Hz and for the radiation group was 7.5Hz. The results show
that the static and dynamic stiffness of the cells decreases after radiation, and less defor-
mation appears in the cells after irradiation. These changes can be due to the breakdown of
membrane chemical bonds and activation of actin fibers after radiation.
REFERENCES (30)
1.
Bao G., Suresh S., 2003, Cell and molecular mechanics of biological materials, Nature Materials, 2, 715-725.
2.
Beaujean F., Caldwell A., Kollár D., Kröninger K., 2011, P-values for model evaluation, Physical Review D, 83, DOI: 10.1103/PhysRevD.83.012004.
3.
Boeddinghaus R., Whyte A., 2008, Current concepts in maxillofacial imaging, European Journal of Radiology, 66, 396-418, DOI: 10.1016/j.ejrad.2007.11.019.
4.
Cerqueira E.D.M.M., Meireles J.R.C., Lopes M.A., Junqueira V.C., Gomes-Filho I.S., Trindade S., Machado-Santelli G.M., 2008, Genotoxic effects of X-rays on keratinized mucosa cells during panoramic dental radiography, Dentomaxillofacial Radiology, 37, 398-403. DOI: 10.1259/dmfr/56848097.
5.
Desprat N., Richert A., Simeon J., Asnacios A., 2005, Creep function of a single living cell, Biophysical Journal, 88, 2224-2233, DOI: 10.1529/biophysj.104.050278.
6.
Dobson J., 2008, Remote control of cellular behaviour with magnetic nanoparticles, Nature Nanotechnology, 3, 139-143, DOI: 10.1038/nnano.2008.39.
7.
Du Y.T., Zhang J., Zheng Q., Li M.X., Liu Y., Zhang B.P., Liu B., Zhang H., Miao G.Y., 2014, Heavy ion and X-ray irradiation alter the cytoskeleton and cytomechanics of cortical neurons, Neural Regeneration Research, 9, 1129-1137, DOI: 10.4103/1673-5374.135315.
8.
Health and Physics: A Grade 12 Manitoba Physics Resource for Health and Radiation Physics, 2009, Canadian Cancer Society.
9.
Ingber D.E., 2002, Mechanical signaling and the cellular response to extracellular matrix in angiogenesis and cardiovascular physiology, Circulation Research, 91, 877-887, DOI: 10.1161/01.RES.0000039537.73816.E5.
10.
Itoh T., Erdmann K.S., Roux A., Habermann B., Werner H., De Camilli P., 2005, Dynamin and the actin cytoskeleton cooperatively regulate plasma membrane invagination by BAR and F-BAR proteins, Developmental Cell, DOI: 10.1016/j.devcel.2005.11.005.
11.
Kalinin A.E., Kajava A.V, Steinert P.M., 2002, Epithelial barrier function: assembly and structural features of the cornified cell envelope, BioEssays, 24,9, 789-800, DOI: 10.1002/bies.10144.
12.
Kanger J.S., Subramaniam V., van Driel R., 2008, Intracellular manipulation of chromatin using magnetic nanoparticles, Chromosome Research, DOI: 10.1007/s10577-008-1239-1.
13.
Kollmannsberger P., Fabry B., 2007, High-force magnetic tweezers with force feedback for biological applications, Review of Scientific Instruments, 78, 1-7, DOI: 10.1063/1.2804771.
14.
Overby D.R., Matthews B.D., Alsberg E., Ingber D.E., 2005, Novel dynamic rheological behavior of individual focal adhesions measured within single cells using electromagnetic pulling cytometry, Acta Biomaterialia, 1, 295-303, DOI: 10.1016/j.actbio.2005.02.003.
15.
Pan Y., Du X., Zhao F., Xu B., 2012, Magnetic nanoparticles for the manipulation of proteins and cells, Chemical Society Reviews, 41, 2912, DOI: 10.1039/c2cs15315g.
16.
Panzetta V., Musellav I., Pugliese M., Piccolo C., Pasqua G., Netti P.A., Fusco S., 2017, Effects of high energy X-rays on cell morphology and functions, ENBENG 2017 – 5th Portuguese Meeting on Bioengineering, Proceedings, DOI: 10.1109/ENBENG.2017.7889448.
17.
Po J.M.C., Kieser J.A., Gallo L.M., T´esenyi A.J., Herbison P., Farella M., 2011, Time-frequency analysis of chewing activity in the natural environment, Journal of Dental Research, 90, 1206-1210, DOI: 10.1177/0022034511416669.
18.
Preethi N., Chikkanarasaiah N., Bethur S.S., 2016, Genotoxic effects of X-rays in buccal mucosal cells in children subjected to dental radiographs, BDJ Open, 2, 16001, DOI: 10.1038/bdjo-pen.2016.1.
19.
Rianna C., Radmacher M., 2016, Cell mechanics as a marker for diseases: Biomedical applications of AFM, AIP Conference Proceedings, 1760, DOI: 10.1063/1.4960276.
20.
Risi R., Manti L., Perna G., Lasalvia M., Capozzi V., Delfino I., Lepore M., 2012, X-ray radiation-induced effects in human mammary epithelial cells investigated by Raman microspectroscopy, SPIE Photonics Europe, 84272E-84272E-10, DOI: 10.1117/12.921389.
21.
Rosenbluth M.J., Lam W.A., Fletcher D.A., 2008, Analyzing cell mechanics in hematologic diseases with microfluidic biophysical flow cytometry, Lab on a Chip, 8, 1062, DOI: 10.1039/b802931h.
22.
Saarikangas J., Zhao H., Lappalainen P., 2010, Regulation of the actin cytoskeleton-plasma membrane interplay by phosphoinositides, Physiological Reviews, DOI: 10.1152/physrev.00036.2009.
23.
Sabanero M., Azor´ın-Vega, J.C., Flores-Villavicencio L.L., Pedro Castruita-Dominguez J., Vallejo M.A., Barbosa-Sabanero G., Cordova-Fraga T., Sosa-Aquino M., 2016, Mammalian cells exposed to ionizing radiation: Structural and biochemical aspects, Applied Radiation and Isotopes, 108, 12-15, DOI: 10.1016/j.apradiso.2015.11.064.
24.
Tanase M,, Biais N, Sheetz M. (2007) Magnetic Tweezers in Cell Biology. Methods in Cell Biology. DOI: 10.1016/S0091-679X(07)83020-2.
25.
Selvaggi L., Salemme M., Vaccaro C., Pesce G., Rusciano G., Sasso A., Campanella C., Carotenuto R., 2010, Multiple-Particle-Tracking to investigate viscoelastic properties in living cells, Methods, 51, 1, DOI: 10.1016/j.ymeth.2009.12.008.
26.
Thomas S., Bolch W., Kao K.J., Bova F., Tran-Son-Tay R., 2003, Effects of X-ray radiation on the rheologic properties of platelets and lymphocytes, Transfusion, 43, 502-508, DOI: 10.1046/j.1537-2995.2003.00360.x.
27.
Valentin J., edit., 2007, The 2007 recommendations of the international commission on radiological protection, Annals of the ICRP, 37, 332.
28.
Verma M., Sonam, Ayub S., 2016, Biological effects of X-rays on X-ray technicians, International Journal of Innovative Research in Science, Engineering and Technology, 18512-18516, DOI: 10.15680/IJIRSET.2016.0510056.
29.
Zhang B., Liu B., Zhang H., Wang J., 2014, Erythrocyte stiffness during morphological remodeling induced by carbon ion radiation, PLOS One, 9, DOI: 10.1371/journal.pone.0112624.
30.
Zheng Q., Liu Y., Zhou H.J., Du Y.T., Zhang B.P., Zhang J., Miao G.Y., Liu B., Zhang H., 2015, X-ray radiation promotes the metastatic potential of tongue squamous cell carcinoma cells via modulation of biomechanical and cytoskeletal properties, Human ans Experimental Toxicology, 34, 894-903, DOI: 10.1177/0960327114561664.
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| eISSN: | 2543-6309 |
| ISSN: | 1429-2955 |
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